hrp conjugated anti rabbit Search Results


anti nlrp3 rabbit polyclonal antibody  (Sangon Biotech)
86
Sangon Biotech anti nlrp3 rabbit polyclonal antibody
A qPCR showed increased TLR4 expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the <t>TLR4/NFκB/NLRP3</t> signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.
Anti Nlrp3 Rabbit Polyclonal Antibody, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+anti+rabbit/anti+conjugated+goat+hrp+igg+rabbit/pmc11742400-321-49-56
Average 86 stars, based on 1 article reviews
anti nlrp3 rabbit polyclonal antibody - by Bioz Stars, 2026-09
86/100 stars
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horseradish peroxidase conjugated goat  (Boster Bio)
96
Boster Bio horseradish peroxidase conjugated goat
A qPCR showed increased TLR4 expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the <t>TLR4/NFκB/NLRP3</t> signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.
Horseradish Peroxidase Conjugated Goat, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+anti+rabbit/HRP+Conjugated+AffiniPure+Goat+Anti-Rabbit+IgG/pm41804010-72-12-19
Average 96 stars, based on 1 article reviews
horseradish peroxidase conjugated goat - by Bioz Stars, 2026-09
96/100 stars
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anti rabbit igg  (Bio-Rad)
99
Bio-Rad anti rabbit igg
A qPCR showed increased TLR4 expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the <t>TLR4/NFκB/NLRP3</t> signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.
Anti Rabbit Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+anti+rabbit/Goat+Anti-Rabbit+IgG+(H+%2B+L)-HRP+Conjugate/bio_rxiv__64898__2026__04__29__721002-147-29-33
Average 99 stars, based on 1 article reviews
anti rabbit igg - by Bioz Stars, 2026-09
99/100 stars
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rabbit anti sheep igg h l hrp conjugate  (Bio-Rad)
94
Bio-Rad rabbit anti sheep igg h l hrp conjugate
A qPCR showed increased TLR4 expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the <t>TLR4/NFκB/NLRP3</t> signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.
Rabbit Anti Sheep Igg H L Hrp Conjugate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+anti+rabbit/Rabbit+Anti-Sheep+IgG+(H%2BL)-HRP+Conjugate/pm34521130-375-4-9
Average 94 stars, based on 1 article reviews
rabbit anti sheep igg h l hrp conjugate - by Bioz Stars, 2026-09
94/100 stars
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immun star goat anti rabbit hrp conjugate  (Bio-Rad)
96
Bio-Rad immun star goat anti rabbit hrp conjugate
A qPCR showed increased TLR4 expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the <t>TLR4/NFκB/NLRP3</t> signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.
Immun Star Goat Anti Rabbit Hrp Conjugate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+anti+rabbit/Immun-Star+Goat+Anti-Rabbit+(GAR)-HRP+Conjugate/pmc13076226-136-8-12
Average 96 stars, based on 1 article reviews
immun star goat anti rabbit hrp conjugate - by Bioz Stars, 2026-09
96/100 stars
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light chain cross adsorbed antibody hrp conjugated  (Bethyl)
95
Bethyl light chain cross adsorbed antibody hrp conjugated
A qPCR showed increased TLR4 expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the <t>TLR4/NFκB/NLRP3</t> signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.
Light Chain Cross Adsorbed Antibody Hrp Conjugated, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+anti+rabbit/Rabbit+Light+Chain+Antibody+HRP+Conjugated/pmc09644419-190-8-13
Average 95 stars, based on 1 article reviews
light chain cross adsorbed antibody hrp conjugated - by Bioz Stars, 2026-09
95/100 stars
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anti rabbit igg hrp conjugate  (Bio-Rad)
96
Bio-Rad anti rabbit igg hrp conjugate
A qPCR showed increased TLR4 expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the <t>TLR4/NFκB/NLRP3</t> signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.
Anti Rabbit Igg Hrp Conjugate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+anti+rabbit/Rabbit+Anti-Goat+IgG+(H%2BL)-HRP+Conjugate/10__1074_slash_jbc__m410487200-92-3-8
Average 96 stars, based on 1 article reviews
anti rabbit igg hrp conjugate - by Bioz Stars, 2026-09
96/100 stars
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ferritin heavy chain polyclonal antibody fth1  (Proteintech)
96
Proteintech ferritin heavy chain polyclonal antibody fth1
FIGURE 2 The aberrant expression of ACSL3 in HCC protects cells from ferroptosis. (A) Genes highly correlated with ACSL3 in HCC were analyzed using Pearson text (B, C) Heatmaps displayed the top 50 genes that are positively and negatively correlated with ACSL3 in HCC. (D) KEGG pathway analysis revealed the correlation between ACSL3 and fatty acid biosynthesis and ferroptosis signaling pathway (E, F) After transfecting ACSL3-oe and control plasmids into PLC/PRF/5 cells, the mRNA and protein expression levels of ACSL4, GPX4, and <t>FTH1</t> were quantified using qPCR and Western blotting. Vinculin protein served as the internal control. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05.
Ferritin Heavy Chain Polyclonal Antibody Fth1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+anti+rabbit/HRP-conjugated+Heavy+chain+of+Rabbit+IgG+Antibody/pm39744125-73-28-34
Average 96 stars, based on 1 article reviews
ferritin heavy chain polyclonal antibody fth1 - by Bioz Stars, 2026-09
96/100 stars
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anti rabbit igg  (Proteintech)
98
Proteintech anti rabbit igg
FIGURE 2 The aberrant expression of ACSL3 in HCC protects cells from ferroptosis. (A) Genes highly correlated with ACSL3 in HCC were analyzed using Pearson text (B, C) Heatmaps displayed the top 50 genes that are positively and negatively correlated with ACSL3 in HCC. (D) KEGG pathway analysis revealed the correlation between ACSL3 and fatty acid biosynthesis and ferroptosis signaling pathway (E, F) After transfecting ACSL3-oe and control plasmids into PLC/PRF/5 cells, the mRNA and protein expression levels of ACSL4, GPX4, and <t>FTH1</t> were quantified using qPCR and Western blotting. Vinculin protein served as the internal control. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05.
Anti Rabbit Igg, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+anti+rabbit/Goat+anti-rabbit+IgG+(H%2BL)%2C+HRP+conjugate/pmc10949762-94-10-12
Average 98 stars, based on 1 article reviews
anti rabbit igg - by Bioz Stars, 2026-09
98/100 stars
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anti rabbit secondary antibodies  (Proteintech)
96
Proteintech anti rabbit secondary antibodies
FIGURE 2 The aberrant expression of ACSL3 in HCC protects cells from ferroptosis. (A) Genes highly correlated with ACSL3 in HCC were analyzed using Pearson text (B, C) Heatmaps displayed the top 50 genes that are positively and negatively correlated with ACSL3 in HCC. (D) KEGG pathway analysis revealed the correlation between ACSL3 and fatty acid biosynthesis and ferroptosis signaling pathway (E, F) After transfecting ACSL3-oe and control plasmids into PLC/PRF/5 cells, the mRNA and protein expression levels of ACSL4, GPX4, and <t>FTH1</t> were quantified using qPCR and Western blotting. Vinculin protein served as the internal control. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05.
Anti Rabbit Secondary Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+anti+rabbit/Rabbit+anti-goat+IgG+(H%2BL)%2C+HRP+conjugate/pmc11630727-234-43-48
Average 96 stars, based on 1 article reviews
anti rabbit secondary antibodies - by Bioz Stars, 2026-09
96/100 stars
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anti rabbit hrp  (Rockland Immunochemicals)
93
Rockland Immunochemicals anti rabbit hrp
FIGURE 2 The aberrant expression of ACSL3 in HCC protects cells from ferroptosis. (A) Genes highly correlated with ACSL3 in HCC were analyzed using Pearson text (B, C) Heatmaps displayed the top 50 genes that are positively and negatively correlated with ACSL3 in HCC. (D) KEGG pathway analysis revealed the correlation between ACSL3 and fatty acid biosynthesis and ferroptosis signaling pathway (E, F) After transfecting ACSL3-oe and control plasmids into PLC/PRF/5 cells, the mRNA and protein expression levels of ACSL4, GPX4, and <t>FTH1</t> were quantified using qPCR and Western blotting. Vinculin protein served as the internal control. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05.
Anti Rabbit Hrp, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+anti+rabbit/Carboxypeptidase+B+Antibody+HRP+Conjugated/pmc09475297-232-13-18
Average 93 stars, based on 1 article reviews
anti rabbit hrp - by Bioz Stars, 2026-09
93/100 stars
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rabbit anti collagen type i ab  (Rockland Immunochemicals)
93
Rockland Immunochemicals rabbit anti collagen type i ab
FIGURE 2 The aberrant expression of ACSL3 in HCC protects cells from ferroptosis. (A) Genes highly correlated with ACSL3 in HCC were analyzed using Pearson text (B, C) Heatmaps displayed the top 50 genes that are positively and negatively correlated with ACSL3 in HCC. (D) KEGG pathway analysis revealed the correlation between ACSL3 and fatty acid biosynthesis and ferroptosis signaling pathway (E, F) After transfecting ACSL3-oe and control plasmids into PLC/PRF/5 cells, the mRNA and protein expression levels of ACSL4, GPX4, and <t>FTH1</t> were quantified using qPCR and Western blotting. Vinculin protein served as the internal control. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05.
Rabbit Anti Collagen Type I Ab, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+anti+rabbit/Collagen+Type+I+Antibody+Peroxidase+Conjugated/pm18250474-110-53-58
Average 93 stars, based on 1 article reviews
rabbit anti collagen type i ab - by Bioz Stars, 2026-09
93/100 stars
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Image Search Results


A qPCR showed increased TLR4 expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.

Journal: Cell Death Discovery

Article Title: pir-hsa-216911 inhibit pyroptosis in hepatocellular carcinoma by suppressing TLR4 initiated GSDMD activation

doi: 10.1038/s41420-024-02285-9

Figure Lengend Snippet: A qPCR showed increased TLR4 expression in the 216911KO cell line. ** p < 0.01 by student T tests. B The diagram presented two pir-hsa-216911 binding sites in 3ʹUTR of TLR4. C The dual luciferase assay revealed that pir-has-216911 could bind both TLR4 binding sites TLR4S1 and TLR4S2. Plasmids carrying TLR4S1 and TLR4S2 generate a higher Firefly/Renilla Luciferase ratio in the 216911KO cell line, which pir-hsa-216911 was absent. **p < 0.01; ***p < 0.001 by student T tests. D TCGA-LIHC survival analysis found low expression of TLR4 to be unfavorable to the survival of HCC patients. E Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway. TLR4, p-p65, and NLRP3 levels were elevated in the 216911KO cell line, which pir-hsa-216911 was absent. F Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway through silencing TLR4. After silencing TLR4 with siRNA, pir-hsa-216911 absent could not elevate p-p65, and NLRP3 levels were elevated in the 216911KO cell line.

Article Snippet: The antibodies used in this study were Cleaved Caspase-1 Rabbit mAb (4199, Cell Signaling Technology, USA), Cleaved Gasdermin D Rabbit mAb (1:2000 dilute; 36425 Cell Signaling Technology, USA), anti-TLR4 rabbit polyclonal antibody (1:500 dilute; D121751, Sangon Biotech, China), anti-RELA (Phospho-Ser536) rabbit polyclonal antibody (1:500 dilute; D155006, Sangon Biotech, China), anti-NLRP3 rabbit polyclonal antibody (1:250 dilute; D120143, Sangon Biotech, China), anti-ACTB rabbit polyclonal antibody (1:6000 dilute; D110001, Sangon Biotech, China), anti-GAPDH rabbit polyclonal antibody (1:4000 dilute; D110016, Sangon Biotech, China), and HRP-conjugated goat anti-rabbit IgG (1:6000 dilute; D110058, Sangon Biotech, China).

Techniques: Expressing, Binding Assay, Luciferase, Western Blot

A The diagram presented the xenograft modeling process. B Euthanized mouse to display the subcutaneous xenografted tumors. C 216911KO group resulted in much smaller tumors in size compared to the control group. D 216911KO group resulted in much smaller tumors in weight compared to the control group. The histogram represents the mean tumor weight of each group. ***p < 0.001 by student T tests. E Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway in vivo. Both TLR4 and p-p65 levels were elevated in tumors formed in the 216911KO group compared to the control group.

Journal: Cell Death Discovery

Article Title: pir-hsa-216911 inhibit pyroptosis in hepatocellular carcinoma by suppressing TLR4 initiated GSDMD activation

doi: 10.1038/s41420-024-02285-9

Figure Lengend Snippet: A The diagram presented the xenograft modeling process. B Euthanized mouse to display the subcutaneous xenografted tumors. C 216911KO group resulted in much smaller tumors in size compared to the control group. D 216911KO group resulted in much smaller tumors in weight compared to the control group. The histogram represents the mean tumor weight of each group. ***p < 0.001 by student T tests. E Western blot analysis revealed pir-hsa-216911 modulating the TLR4/NFκB/NLRP3 signaling pathway in vivo. Both TLR4 and p-p65 levels were elevated in tumors formed in the 216911KO group compared to the control group.

Article Snippet: The antibodies used in this study were Cleaved Caspase-1 Rabbit mAb (4199, Cell Signaling Technology, USA), Cleaved Gasdermin D Rabbit mAb (1:2000 dilute; 36425 Cell Signaling Technology, USA), anti-TLR4 rabbit polyclonal antibody (1:500 dilute; D121751, Sangon Biotech, China), anti-RELA (Phospho-Ser536) rabbit polyclonal antibody (1:500 dilute; D155006, Sangon Biotech, China), anti-NLRP3 rabbit polyclonal antibody (1:250 dilute; D120143, Sangon Biotech, China), anti-ACTB rabbit polyclonal antibody (1:6000 dilute; D110001, Sangon Biotech, China), anti-GAPDH rabbit polyclonal antibody (1:4000 dilute; D110016, Sangon Biotech, China), and HRP-conjugated goat anti-rabbit IgG (1:6000 dilute; D110058, Sangon Biotech, China).

Techniques: Control, Western Blot, In Vivo

FIGURE 2 The aberrant expression of ACSL3 in HCC protects cells from ferroptosis. (A) Genes highly correlated with ACSL3 in HCC were analyzed using Pearson text (B, C) Heatmaps displayed the top 50 genes that are positively and negatively correlated with ACSL3 in HCC. (D) KEGG pathway analysis revealed the correlation between ACSL3 and fatty acid biosynthesis and ferroptosis signaling pathway (E, F) After transfecting ACSL3-oe and control plasmids into PLC/PRF/5 cells, the mRNA and protein expression levels of ACSL4, GPX4, and FTH1 were quantified using qPCR and Western blotting. Vinculin protein served as the internal control. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05.

Journal: Frontiers in pharmacology

Article Title: Transcription factor MEF2D regulates aberrant expression of ACSL3 and enhances sorafenib resistance by inhibiting ferroptosis in HCC.

doi: 10.3389/fphar.2024.1464852

Figure Lengend Snippet: FIGURE 2 The aberrant expression of ACSL3 in HCC protects cells from ferroptosis. (A) Genes highly correlated with ACSL3 in HCC were analyzed using Pearson text (B, C) Heatmaps displayed the top 50 genes that are positively and negatively correlated with ACSL3 in HCC. (D) KEGG pathway analysis revealed the correlation between ACSL3 and fatty acid biosynthesis and ferroptosis signaling pathway (E, F) After transfecting ACSL3-oe and control plasmids into PLC/PRF/5 cells, the mRNA and protein expression levels of ACSL4, GPX4, and FTH1 were quantified using qPCR and Western blotting. Vinculin protein served as the internal control. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05.

Article Snippet: ACSL3 antibody (Santa cruz, 1:100, Cat# sc-166374), ACSL4 Rabbit pAb (ABclonal, 1:1,000, Cat#A6826), MEF2D antibody (Santa cruz, 1:500, Cat# sc-271153), GPX4Monoclonal antibody (proteintech, 1:1,000, Cat No.: 67763-1- Ig), Ferritin heavy chain Polyclonal antibody (FTH1) (proteintech, 1:1,000, Cat No.: 11682-1-AP), Vinculin Monoclonal antibody (proteintech, 1:10,000, Cat No.: 66305-1-Ig).

Techniques: Expressing, Control, Western Blot